פרופ' גלבוע-גרבר נחמה

פרופ'
פרופסור אמריטוס
פרופ'
דוא"ל: 
טלפון: 
פקס : 
מיקום: 
שעות קבלה: 
10:00 - 16:00

קורות חיים

 

Education

1959, MSc (cum laude), in Microbiology (Biochemistry and Parasitology)Hebrew University, Jerusalem

1963, PhD, in Microbiology and Immunohematology, Hebrew University, Jerusalem (Medical School)

1963-1967, Post-doctorate, in Biochemistry (with Prof. J. Mager).
Subject: The enzyme sulfite reductase of Escherichia coli
Hebrew University, Jerusalem (Medical School)

Positions

2004-present, PROFESSOR EMERITUS
The Mina and Everard Goodman Faculty of Life Sciences
Bar-Ilan University, Ramat-Gan, Israel

1986-2004, FULL PROFESSOR, Biochemistry
Dept. of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

1980-1986, ASSOCIATE PROFESSOR, Biochemistry
Dept. of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

1972-1980, SENIOR LECTURER, Biochemistry
Dept. of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

1969-1972, LECTURER, Biochemistry
Dept. of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

1963-1967, INSTRUCTOR, Cell Biochemistry and Clinical Biochemistry
The Hebrew University and Hadassah Medical School, Jerusalem

1958-1963, ASSISTANT INSTRUCTOR
Hematology, Immunohematology and Clinical Microbiology
The Hebrew University and Hadassah Medical School, Jerusalem

 

Academic Administrative Positions

2001-2006, Member, ISM Prize Committee (Israel Society for Microbiology)

2001-2004, Member, University Constitution Committee

1999-2001, Member of the University Student Admission Committee

1997-2000, Member of the Department of Life Sciences Committee for Graduate Students

1996-2000, Deputy Head of the University Committee for Student Scholarships

1996-1997, Chairperson (head) of the organizing committee of the

Annual Meeting of the Israel Society for Microbiology, Ramat-Gan, 1997

1996, Member of the Organizing Committee of the 8th IUMS International Union of Microbial Societies Congress of Bacteriology and Applied Microbiology, Jerusalem, 1996

1996, Member of the Organizing Committee for Bat-Sheva Seminar

“Toward anti-adhesion therapy of microbial diseases”, Zichron Yaakov, 1996

1995-1997, Secretary of Israel Society for Microbiology

1995-1997, Member of the Committee of Israel Society for Microbiology

1993-1995, Deputy Head of the University Disciplinary Committee

1989-1993, Member of the University Teaching Committee

1990-1991, Member of Committee of International Lectin Society

1989-1991, Head of Student Admission Committee, Bar-Ilan University

1987-1988, Member of the committee of the Israel National Academy of Life Sciences Foundation (headed by Prof. A. Dvir)

1986-2004, Member of Senate, Bar-Ilan University

1980-1989, Deputy Head of Student Admission Committee, Bar-Ilan University

1983-1985, Responsibility for Seminar Lectures of Dept. of Life Sciences

1982-1983, Head of the Dept. of Life Sciences, Bar-Ilan University

1981-1982, Deputy Head of the Dept. of Life Sciences, Bar-Ilan University

1980-1982, Head of Teaching Committee, Dept. of Life Sciences, Bar-Ilan University

1978-1982, Member of “VATAT” Committees for Instrument Budgeting

(headed by Prof. J. Birk, Prof. A. Zamir, etc.)

1976-1984, Member of the Committee of Israel Biochemical Society

1970-1980, Member of the Teaching Committee, Dept. of Life Sciences, Bar-Ilan University

 

Membership in Organizing Committees of Scientific Meetings

1983, Organization of a short (afternoon) Meeting on Oncogenes

(with Prof. George Klein and Prof. Benjamin Geiger as lecturers)

in Bar-Ilan University, related to the Israel Society for Biochemistry (being a member of its committee).

 1984, Member of the Organizing Committee of the Annual Meeting of the Israel Society for Microbiology  in Bar-Ilan University

1996, Member of the Organizing Committee for Bat-Sheva Seminar “Toward anti-adhesion therapy of microbial diseases”, Zichron Yaakov, 1996

1996, Member of the Organizing Committee of the 8th IUMS International Union of Microbial Societies Congress of Bacteriology and Applied Microbiology, Jerusalem, 1996

1996-1997, Chairperson (head) of the Organizing Committee of the Annual Meeting of the Israel Society for Microbiology, Ramat-Gan, 1997

2004-2005, Member of the Organizing Committee of the Annual Meeting of the Israel Society for Microbiology, Tel-Aviv, 2005

Honors

1962, Medical School, Hebrew University, Jerusalem, for PhD thesis

1976, Israel Society for Microbiology, for research on Pseudomonas aeruginosa cholinesterase and hemagglutinins

1988, Council of Women's Organizations in Israel: to outstanding women who have registered exceptional accomplishments in their professions - as Israel marks its Fortieth Anniversary

2004, Outstanding Lecturer Award, Faculty of Life Sciences, Bar-Ilan University

 

 

 

 

 

 

 

 

פרסומים

Recent articles

Zinger-Yosovich, K., Sudakevitz, D., Imberty, A., Garber, N.C. and Gilboa-Garber, N. Production and properties of native Chromobacterium violacearum fucose-binding lectin  (CV-IIL) compared to homologous lectins of Pseudomonas aeruginosa (PA-IIL) and Ralstonia solanacearum (RS-IIL). Microbiology 152: 457-463, 2006.

Gilboa-Garber, N., Sudakevitz, D., Levene, C., Rahimi-Levene, N. and Yahalom, V. H-deficient Bombay and para-Bombay erythrocytes are most strongly agglutinated by the galactophilic lectins of Aplysia and Pseudomonas aeruginosa that detect I and P1 antigens. Immunohematology 22: 15-22, 2006.

Pokorná, M., Cioci, G., Perret, S., Rebuffet, E., Kostlànová, N., Adam, J., Gilboa-Garber, N., Mitchell, E.P., Imberty, A. and Wimmerová, M. Unusual entropy driven affinity of Chromobacterium violaceum lectin CV-IIL towards fucose and mannose. Biochemistry 45: 7501-7510, 2006.

Wu, A.M., Wu, J.H.; Singh, T., Liu, J.-H., Tsai, M.-S. and Gilboa-Garber, N. Interactions of the fucose-specific Pseudomonas aeruginosa  lectin (PA-IIL) with mammalian glycoconjugates bearing polyvalent Lewisa and ABH blood group glycotopes. Biochimie 88: 1479-1492, 2006.

Katri, N. and Gilboa-Garber, N. Ethanol effects on Pseudomonas aeruginosa  lectin, protease, hemolysin, pyocyanin, autoinducer and phosphatase levels depending on medium composition and choline presence. Curr. Microbiol. 54: 296-301, 2007.

Lerrer, B., Zinger-Yosovich, K.D. Avrahami, B. and Gilboa-Garber, N. Honey and royal jelly, like human milk, abrogate lectin-dependent infection-preceding Pseudomonas aeruginosa adhesion. ISME J (Nature group) 1: 149-155, 2007.

Katri, N., Garber, N.C., Kilfin, G. and Gilboa-Garber, N. Abrogation of the resistance of choline-induced Pseudomonas aeruginosa virulence to sub-MIC erythromycin by ethanol. ISME J (Nature group) 2: 1243-1246, 2008.

Wu, A.M., Wu, J.H., Singh, T., Singha, B., Sudakevitz, and Gilboa-Garber, N. Multivalent human blood group ABH and Lewis glycotopes are key recognition factors for a LFuc>Man binding lectin from phytopathogenic Ralstonia solanacearum. Biochem.Biophys. Acta 1790: 249-259, 2009.

Gilboa-Garber, N.  and Zinger-Yosovich, K.D. Comparison of the antibacterial adhesion activities of honey and royal jelly in blocking lectins of the soil-borne pathogens Pseudomonas aeruginosa, Chromobacterium violaceum and Ralstonia solanacearum  to those of milk. Journal of ApiProduct and ApiMedical Science 1, 82-89, 2009.

Zinger–Yosovich, K.D., and Gilboa-Garber, N. Blocking of Pseudomonas aeruginosa and Ralstonia solanacearum lectins by plant and microbial branched polysaccharides used as  food additives. J. Agric. Food Chem. 57: 6908-6913, 2009.

Zinger-Yosovich, K.D., Sudakevitz, D., Iluz, D. and Gilboa-Garber, N. Blocking of Pseudomonas aeruginosa and Chromobacterium violaceum lectins by diverse mammalian milks. J. Dairy Sci., 93: 473-482, 2010.

Wu, A. M., Gong, Y- P. Li, C-C and Gilboa-Garber, N. Duality of the carbohydrate-recognition system of Pseudomonas aeruginosa-II lectin (PA-IIL).  FEBS Lett. 584: 2371-2375, 2010.

Iluz, D., Hoffman, M., Gilboa-Garber, N. and Amar, Z. Medicinal properties of Commiphora gileadensis. African J. Pharmac. Pharmacol. 4: 516-520, 2010.

Zinger-Yosovich, K.D., Sudakevitz, D., Iluz, D. and Gilboa-Garber, N. Analyses of diverse mammals' milk and lactoferrin glycans using five pathogenic bacterial lectins. Food Chem. 124: 1335-1342, 2011.

Gilboa-Garber, N., Zinger-Yosovich, K.D., Sudakevitz, D., Lerrer, B., Imberty, A., Wimmerova, M., Wu, A.M. and Garber, N.C. The five bacterial lectins: PA-IL, PA-IIL, RSL, RS-IIL, and CV-IIL- interactions with diverse animal cells and glycoproteins. Chapter 9 in The Molecular Immunology of Complex Carbohydrates-3 (edited by A.M.Wu) In Advances in Experimental Medicine and Biology 705: 155-211, 2011.

Garber, N.C., Zinger-Yosovich, K.D., Sudakevitz, D., Axelrad, I. and Gilboa-Garber, N. Regulation of lectin production by the human pathogens Pseudomonas aeruginosa and Chromobacterium violaceum: effects of choline, trehalose and ethanol. Chapter 11 in  The Molecular Immunology of Complex Carbohydrates-3  (edited by A.M.Wu) In Advances in Experimental Medicine and Biology 705: 229-256, 2011.

 

Industrial Products and Patents

A vaccine against Pseudomonas aeruginosa based on the lectin of the bacterium (Israeli Patent, 1980)

Erythrina corallodendron lectin (Sigma, p. 1587, 1990, p. 1955, 1997)

Pseudomonas aeruginosa PA-I lectin (Sigma, p. 1596, 1990; p. 1964, 1997)

 

 

 

 

מחקר

Scientific Contributions

Pioneer study on human leukocyte and platelet antigens [Nature 1961] - 4 citations

Devising a widely currently used sulfide determination method [Anal. Biochem. 1981] - 118 citations 

Pioneer discovery and study of the opportunistic pathogen Pseudomonas aeruginosa cholinesterase [Biochim. Biophys. Acta 1973] - 29 citations

Pioneer discovery, isolation by affinity chromatography and studies of the pathogenic bacterial  lectins PA-IL and PA-IIL of P. aeruginosa (LecA, galactophilic and Lec B, fucose and mannose-binding) which contribute to its virulence by adhering to the victim host cell saccahrides  [FEBS Lett. 1972;  Can. J. Microbiol., 1977; Meth. Enzymol., 1982, etc.] - 280 citations

♦♦♦♦

Among the works that cite our above discoveries of PA-IL and PA-IIL lectins and their genes there are several most advanced recent important reports, which use them for the following 

a)  Nanotechnology-based projects where single walled nanotubes bearing these lectins as biosensors are used for nanoelectronic detection of biomolecules replacing optical sensors [Vadala, H.  et al., NanoLett. 2011]

b)  NASA-supported projects (performed under spaceflight conditions in NASA-Space Center, Houston, Texas) aimed to examine the danger of increased pathogenic bacterial (P. aeruginosa) virulence during spaceflight missions. In these studies the two P. aeruginosa lectin genes (also originally isolated by us) were found to be the major P. aeruginosa genes induced under the spaceflight conditions [Crabbe et al., headed by Cheryl A Nickerson, published in Appl. Environ. Microbiol.  2011].

♦♦♦♦

First demonstrations of bacterial lectin (PA-IL and PA-IIL) mitogenicity for human peripheral lymphocytes and stimulation of Tetrahymena cells [FEMS Microbiol Lett. 1979; J. Protozoology 1980; FEBS Lett. 1987] - 55 citations

Discovery of Erythrina corallodendron ECorL lectin, its mitogenicity and H-blood group specificity [Can. J. Biochem. 1981; Lectins 1994] - 30 citations

Proving that the productions of P. aeruginosa lectins and its other virulence factors are co-regulated [Lectins 1983] - 13 citations

Discovery of Tetraclita squamosa (barnacle) chitin–binding lectin [Mar. Biol. Lett. 1983] - 3 citations

Discovery and studies of Aplysia (giant marine snail) gonad lectin (AGL) - the first galacturonic ac.-specific lectin, its applications for  histochemical and cytochemical studies, for anti - tumoral effects and for isolation of E. coli strains bearing T-antigen-like antigens that cross react with the related cancer antigens, and its interactions with diverse glycans [FEBS Lett. 1985; Anticancer Res.1987; J. Histochem. Cytochem. 1988; J. Biol. Chem. 2000; FEMS Immunol. Med. Microbiol. 2001] - 123 citations

Application of PA-IL (LecA) and PA-IIL (LecB) mix as an effective vaccine against P. aeruginosa [FEMS Microbiol Lett. 1987] - 4 citations

Discovery and study of Ulva lactuca fucose-binding H- and Le-specific lectin ULL [Dev. Comp. Immunol. 1988; Lectins 1994] - 14 citations

Describing PA-IL and PA-IIL lectin interactions with the common ABO(H) and P blood group system {Pk and P1) glycosphingolipid antigens – a potential contribution to the broad spectrum adherence of P. aeruginosa to human tissues in secondary infections (Glycoconjugate J. 1994) - 38 citations

Forwarding the lectin-enzyme functional postulation [FEMS Microbiol. Rev. 1989] - 35 citations

Isolation of the PA-IL and PA-IIL genes (lecA and lecB), elaboration of their sequences and their expression in E. coli [J. Biol. Chem. 1992; BBA 1994; FEMS Immunol. Med. Microbiol. 2000] - 79 citations

First usage of lectins for studies of halophilic archaea surface saccharides.  [FEMS Microbiol. 1998] - 8 citations

Demonstrtion of P. aeruginosa quorum-sensing (QS) autoinducer-linked lectin production sensitivity to sub-MIC erythromycin [Chemotherapy, 1999] - 35 citations

Discovery and studies of Ralstonia solanacearum fucophilic lectin RSL [J. Biochem. 2002; J. Biol. Chem. 2005] - 42 citations

Collaborated study (with A. Imberty et al., Grenoble) of the 3-D crystal structure of PA-IIL [Nature Struct. Biol. 2002] – 91 citations and its strong interacions [Proteins 2005] - 31 citations

Collaborated study (with A. Imberty et al., Grenoble) of the 3-D crystal structure of PA-IL [FEBS lett. 2003]  - 41 citations

The later 2 works led to unveiling of the molecular basis of host-glycan recognition by P. aeruginosa lectins [Microb. Infect., 2004] - 54 citations

Discovery and studies (collaborative) of the aggressive plant pathogen Ralstonia solanacearum mannophilic lectin RS-IIL and its 3D structure interactions [Mol. Microbiol. 2004] - 21 citations

Discovery and studies (collaborative) of the opportunistic pathogen Chromobacterium violaceum fucophilic lectin CV-IIL and its 3D structure interactions [Microbiology-SGM, 2006; Biochemistry 2006] - 16 citations

Studies of the 5 pathogenic bacterial lectins (PA-IL, PA-IIL, RSL, RS-IIL and CV-IIL) blocking by diverse milks, human body fluids, avian egg whites, honey and royal jelly, plant seeds and fruits, providing protection to sensitive embryos, neonates and organs against infections

Applications of these lectins, tagged by peroxidase, for detecting interacting glycoproteins that exist in these substances by staining their Western blots [FEMS Immunol. Med. Microbiol. 2001; Can. J. Microbiol. 2001; Electrophoresis 2003; ISME J. 2007. J. ApiProd. ApiMed. Sci. 2009).

The total number of citations documented in ISI WOS of Knowledge search of Gilboa-Garber's (recent) and Gilboagarber's (earlier) publications exceeds 1600

 

Lectins discovered and studied in Prof. N. Gilboa-Garber's laboratory can be viewed here