פרופ' פרל-טרבס רפאל

פרופ'
פרופסור חבר
דרושים סטודנטים לMSc ו PhD למחקר לפרטים ניתן לפנות לפרופ' רפי פרל טרבס
פרופ' רפאל פרל-טרבס
דוא"ל: 
טלפון: 
מיקום: 

קורות חיים

Education

 

1982, BSc, with honors, Faculty of Agriculture, Field Crop Department, Hebrew University, Rehovot, Israel

 

1985, MSc, Plant Genetics Department, Weizmann Institute of Science, Rehovot, Israel

 

1990, PhD, Plant Genetics Department, Weizmann Institute of Science, Rehovot, Israel

 

1990-1992, Postdoctoral research, Cornell University, Ithaca NY, USA

 

Positions

 

2007-present, Associate Professor

Plant Molecular Genetics and Development

The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University,

Ramat-Gan, Israel

 

1999-2007, Senior Lecturer

Plant Molecular Genetics and Development

The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University,

Ramat-Gan, Israel

 

2002, Sabbatical Fellow

Plant/pathogen interaction

Plant Industry Division, CSIRO, Wembley (Perth) West Australia

 

1993-1999, Lecturer

Plant Molecular Genetics and Development

Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

 

 

Public Scientific Activities

 

2005-2009, Treasurer of the Israeli Society of Plant Sciences

 

2008, Scientific Board of the Cucurbitaceae 2008 International Conference

Avignon, France

 

2006-2007, Head of the Israeli Society of Plant Sciences

 

2005-2007, Evaluation Committee for Field and Garden Crops, BARD

 

2001-2005, Evaluation and Steering Committee for Agriculture according to Halachic Law, Chief Scientist’s Office of the Israeli Ministry of Agriculture

 

1999-2000, Scientific Board organizing the Cucurbitaceae 2000 International Conference, Ma’ale Ha’Hamisha, March 2000

 

1994-1996, Evaluation Committee for Field and Garden Crops, BARD and Chief Scientist's Office of the Israeli Ministry of Agriculture

 

 

Past and Present Members of our Group (*=presently in the lab)

 

*Dr. Irina Kovalski, New immigrant Scientist and lab assistant, 1993-present;

Thesis/Project: Development and mapping of RAPD markers in melons

 

Dr. Leah Silberstein, Postdoctoral Fellow, 1993-2002

Thesis/Project: Development and mapping of RFLP markers in melons

 

Dr. Huang Ruguo, Postdoctoral Fellow - part time, 1995

Thesis/Project: RAPD and RFLP genotyping of pathogenic fungi and melon varieties

 

Dr. Xiang Yu, Postdoctoral Fellow, 1995

Thesis/Project: Cloning of sex related genes in cucumber

 

Dr. Naama Kessler, Laboratory assistant part-time, 1998-1999

Thesis/Project: Cloning characterization of male specific cDNAs in cucumber

 

Anat Kahana, Ph.D., 1998 (joint supervision with Prof. Y. Cohen)

Thesis: Molecular characterization of sex expression in cucumber

 

Naomi Rosenmann, M.Sc., 1996

Thesis: Isolation and characterization of homeotic genes in cucumber

 

Asya Stepansky,  M.Sc., 1998

Thesis: Molecular and phenotypic variation in the melon germplasm

 

Yariv Brotman, M.Sc., 2000

Thesis: Cloning and linkage analysis of resistance-gene homologues from melon

 

Anat Goldberg, M.Sc., 2002

Thesis: Genes expressed in buds of C. sativus

 

Talia Saraf-Levy, Ph.D., 2003

Thesis: Role of ethylene perception in cucumber sex expression

 

Anat Goldberg, M.Sc., 2002

Thesis: Genes expressed in buds of C. sativus

 

Dr. An Lizheé, Postdoctoral Fellow, 2000

Project: Genetic transformation of C. sativus

 

Salih Kafkas, Ph.D. student at Chukrova University, Turkey. Joint supervision with Prof. Selim Cetiner, performed a two-year project at Bar-Ilan University, 1998 – 2000

Project: Wild Pistacia species from Turkey: sex expression, genetic diversity and evaluation as rootstock for P. vera

 

Dr. Premanand Rajagopalan, Postdoctoral Fellow, 2001-2003

Project: Genetic transformation of Cucumis sativus

 

Yariv Brotman, Ph.D., 2004

Thesis: Mapping of resistance-genes from melon

 

Mor Grinberg, M.Sc., 2003

(Joint supervision Dr. Victoria Sorozker and Dr. Eric Palewski, A.R.O.)

Thesis: Interaction between cucumber and the broad mite

 

Michal Segal, M.Sc., 2003

Thesis: Pto kinase homologs in melon

 

Isaac Goldenberg, M.Sc., 2004

Thesis: Mapping resistance genes in melon

 

Ran Herman, Ph.D., 2008

Thesis: Inheritance & mapping of Fusarium race 1,2 resistance in melon

 

Zvi Zvirin, M.Sc., 2006

Thesis: Mapping Fusarium resistance in melon

 

Anat Hendelman, M.Sc., 2006

Thesis: Expression of microRNA in developing cucumber flowers

 

Avital Baniel, M.Sc., 2007

Thesis: Control of fruit set in Cucumis

 

Valentina Davidovich, M.Sc. student, 2010

Thesis: Engineered hormonal changes in cucumber

 

Ohad Yogev, M.Sc., 2009

Thesis: Melon root transformation 

 

Mor Grinberg, Ph.D., 2009

(Joint supervision Dr. Victoria Sorocker, A.R.O.)

Thesis: Interaction between cucumber and the broad mite

 

Isaac Goldenberg, Ph.D., 2009

(Joint supervision Dr. Nurit Katzir, A.R.O.)

Thesis: Positional cloning of resistance genes in melon

 

Avida Shoham, M.Sc. student, 2010

(Joint supervision Dr. Ron Cohen, A.R.O.)

Thesis: Partial resistance of melons to Fusarium oxysporum

 

*Toiba Safronova, M.Sc. student, 2008-

(Joint supervision Dr. Victoria Sorocker, A.R.O.)

Thesis: Interaction between tomato, the broad mite and whiteflies

 

*Dikla Elul, M.Sc. student, 2009-

(Joint supervision Dr. Victoria Sorocker, A.R.O.)

Thesis: Interaction between tomato, the broad mite and whiteflies

 

Rina Zagouri, M.Sc. student, 2010

Thesis: Cloning of the sex determining gene androecious in cucumber

 

Michael Normantovich, M.Sc. student, 2010

Thesis: Composite melon plants expressing Fusarium resistance

 

Golan Miller, M.Sc. student, 2011

Thesis: Control of fruit set in Cucumis

 

Yulia Goltsev, M.Sc. student, 2011

Thesis: Hormonal control of fruit set in Cucumis

 

*Anastacia Stovbun, M.Sc student, 2013

Project: Positional cloning of resistance genes in melon

 

Arie Amitzur , M.Sc student, 2012

Thesis: Mapping of the parthenocarpy trait in cucumber

 

 *Michael Normantovich, Ph.D. student, 2010-

Thesis: Fusarium and PRSV resistance genes in melon  

 

*Golan Miller, Ph.D. student, 2012 -

Thesis: Fusarium-melon interaction

 

*Yulia Goltsev, Ph.D. student, 2012 -

Thesis: Hormonal control of fruit set in Cucumis

 

*Arie Amitzur , Ph.D. student, 2013 -

Thesis: Potyvirus resistance genes in melon

 

 

In addition, I have provided academic supervision to graduate students who performed their research at the Agricultural Research Organization:

 

Esther Aftergoot, M.Sc. (co-supervisor Dr. Ilan Paran), 1997-1999

Hila Spanov, M.Sc. (co-supervisor Dr. Yedidya Gafni), 2001-2003

Natalia Gerchikov, M.Sc. (co-supervisor Dr. Idit Ginzburg), 2002-2005

Tal Twito, Ph.D. (A.R.O. and Hebrew University, co-supervisors Dr. Uri Lavi and Prof. Yossef Hillel), 2002-2009

Noga Yaakov, M.Sc. (co-supervisor Dr. Yedidya Gafni), 2003-2006

Dvora Schwarzberg, Ph.D. (co-supervisor Dr. David Granot), 2004-2008

Sarit Faraj-Barhom, Ph.D. (co-supervisor Dr. Amnon Lers), 2004-2009

Efrat Saar-Glick, Ph.D. (co-supervisor Dr. Yedidya Gafni), 2004-2009

Pinhas Kritz, M.Sc. (co-supervisor Dr. Idit Ginzburg), 2005-2007

Yelena Ivanov, Ph.D. (co-supervisor Dr. David Kenigsbuch), 2006-

Shahar Oheli, M.Sc. (co-supervisor Dr. Oz Barazani), 2008-2010

Anat Krepkanovich, M.Sc. (co-supervisor Dr. Yuval Cohen), 2009-2010

Nir Hanin, M.Sc. (co-supervisor Dr. Oz Barazani), 2013

Amalia Aboudi, Ph.D. (co-supervisor Dr. Yedidya Gafni), 2009-

Amir Dekel, M.Sc. (co-supervisor Dr. Victoria Sorocker), 2011-

תקשורת

 תמונות נוספות של רפי: 1234.

תמונות של הקבוצה שלנו: 

19952000200220042004b2004c2010a2010b2011a2011b

2011c,2013.

איורים של הקבוצה שלנו:

IrinaPremYariv&Zachi1Yariv&Zachi2ZachiMor1Mor2Michal

RaniAvitalAnatHAvidaGolanYula.

 

 תמונות משפחתיות:

Ithaca 1992Yair's Bar-Mizva 2001Alps 2003Tamar's Bat-Mizva 2004Perth 2003abcdef , Cairns 2003Perl's 50th anniversary 2006abBeijing 2006abBadaling 2006Yunnan 2006abcdNegev Desert  2007abCroatia 2008Metz 2008Jordan Valley 2008Paris 2008Noam 2008David 2007abYair 2008David 2009,Torino2012abcdRoma 2012Nova Scotia 2010abcdeHanukka 2012Succot 2012Lazarus Wedding 2012abc,  Tamar enrolls 2012ab, Japan 2012abcdef,ghijNoam Bar Mizva abcd.

פרסומים

 

 

Journals

 

1. Perl-Treves, R. and Galun, E. (1985) The Cucumis plastome: physical map, intrageneric variation and phylogenetic relationship. Theor. Appl. Genet. 71: 417-429.

 

2. Perl-Treves, R., Zamir, D., Navot, N. and Galun, E. (1985) Phylogeny of Cucumis based on isozyme variability and its comparison with plastome phylogeny. Theor. Appl. Genet. 71: 430-436.

 

3. Perl-Treves, R., Nacmias, B., Aviv, D., Zeelon, E.P. and Galun, E. (1988) Isolation of two cDNA clones from tomato containing two different superoxide dismutase sequences. Plant Mol. Biol. 11: 609--623.

 

4. Perl-Treves, R., Abu-Abied, M., Magal, N., Galun, E. and Zamir, D. (1990) Genetic mapping of tomato cDNA clones encoding the chloroplastic and cytosolicisozymes of superoxide dismutase. Biochem. Genet. 28: 543-552.

 

5. Perl-Treves, R. and Galun, E. (1991) The tomato Cu, Zn superoxide dismutase genes are developmentally regulated and respond to light and stress. Plant Mol. Biol.17: 745-760.

 

6. Perl, A., Perl-Treves, R., Galili, S., Aviv, D., Shalgi, E., Malkin, S. and Galun, E. (1992) Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu, Zn superoxide dismutases. Theor. Appl. Genet. 85: 568-576.

 

7. Perl-Treves, R., Howlett, B. and Nasrallah, M.E. (1993) Self-incompatibility related glycoproteins of Brassica are produced and secreted by transgenic tobacco cell cultures. Plant Science 92: 99-110.

 

8. Abadi, R., Perl-Treves, R. and Levy, Y. (1996) Molecular variability among Exserohilum turcicum isolates using RAPD (Random Amplified Polymorphic DNA). Can. J. Plant Pathol. 18: 29-34.

 

9. Huang R., Galperin M., Levy Y., Perl-Treves R. (1997) Genetic variation of Fusarium moniliforme detected by random amplified polymorphic DNA markers. PlantPathol. 46: 871-881.

 

10. Mizrahi L., Achituv Y., Katcoff D. and Perl-Treves R. (1998) Phylogenetic position of Ibla (Cirripedia; Thoracica) based on 18S r DNA sequence analysis. J. Crustacean Biol. 18: 363-368.

 

11. Perl-Treves R., Kahana A., Rosenmann N., Yu X., Silberstein L. (1998) Expression of multiple Agamous-like genes in male and female flowers of cucumber (Cucumissativus L.). Plant Cell Physiol. 39: 701-710.

 

12. Perl-Treves R., Kahana A., Korach T., Kessler N. (1998) Cloning of three cDNAs encoding 1-aminocycloproane-1-carboxylate oxidases from cucumber floral buds (accession Nos. AF033581, AF033582 and AF033583) (PGR98-037). Plant Physiol. 116: 1192.

 

13. Silberstein L., Kovalski I., Huang R., Anagnostou K., Kyle M.M. andPerl-Treves R. (1999) Molecular variation in Cucumis melo as revealed by RFLP and RAPD markers. Scientia Horticulturae 79: 101-111.

 

14. Stepansky A., Kovalski I., Schaffer A.A., Perl-Treves R. (1999) Variation in sugar levels and invertase activity in mature fruit representing a broad spectrum ofCucumis melo genotypes. Genet. Resources Crop Evol. 46: 53-62.

 

15. Stepansky A, Kovalski I, Perl-Treves R. (1999) Intraspecific classification of Cucumis melo based on morphological and molecular evaluation. Plant Systematics and Evolution 217: 313-333.

 

16. Kahana A., Silberstein L., Kessler N., Goldstein R.S., Perl-Treves R.(1999) Expression of cucumber ACC oxidases differs among sex genotypes and sex phases. Plant Mol. Biol. 41: 517-528.

 

17. Perl-Treves R., Mizrahi L, Katcoff D.J. and Achituv Y. (2000) Elucidation of the phylogenetic relationship of three cirripeds, Verruca, Paralepas and Dendrogaster(Cirripedia; Thoracica) based on 18S r DNA sequence. J. Crustacean Biol. 20: 385-392.

 

18. Mokadi, O., Mizrahi L., Perl-Treves R. and Achituv Y. (2000) The different morphs of Chtamalus anisopoma - a phenotypic response? Direct molecular evidence. J. Exp. Marine Biol. Ecol. 243: 295-304.

 

19. Anagnostou K., Kyle M. and Perl-Treves R. (2000) Inheritance and linkage analysis of resistance to zucchini yellow mosaic virus, watermelon mosaic virus, papayaringspot virus and powdery mildew in melon. Euphytica 116: 265-270.

 

20. Kafkas S., Perl-Treves R. and Kaska N. (2000) Unusual Pistacia atlantica trees in the Yunt Mountains of the Manisa Province of Turkey. Isr. J. Plant Sci. 48: 277-280.

 

21. Klingler J., Kovalski I., Silberstein L., Thompson G.A. and Perl-Treves R. (2001) Mapping of cotton-melon aphid resistance in melon. J. Am. Soc. Hortic. Sci. 126: 56-63.

 

22. Kafkas S and Perl-Treves R. (2001) Morphological and molecular phylogeny of Pistacia species in Turkey. Theor. Appl. Genet. 102: 908-915.

 

23. Kafkas S., Cetiner MS and Perl-Treves R. (2001) Development of sex-associated RAPD markers in wild Pistacia species. J. Hortic Sci. Technol. 76: 242-246.

 

24. Kafkas S. and Perl-Treves R. (2002) Inter-specific relationships in the genus Pistacia L. (Anacardiaceae) based on RAPD fingerprints. HortScience 37: 168-171.

 

25. Kafkas S, Kafkas E and Perl-Treves R. (2002) Morphological diversity and a germplasm survey of wild Pistacia species in Turkey. Genet. Resources Crop Evol. 49: 261-270.

 

26. Brotman Y, Silberstein L, Kovalski I, Perin C, Dogimont C, Pitrat M, Klingler J, Thompson G and Perl-Treves R. (2002) Resistance gene homologues in melon and their linkage to genetic loci conferring disease and pest resistance. Theor. Appl. Genet. 104: 1055-1065.

 

27. Karaco S., Achituv Y., Perl-Treves R. and Katcoff D. (2002) Asterina burtoni (Asteroidea; Echinodermata) in the Mediterranean and the Red Sea: does asexual reproduction ability enhance colonization? Mar. Ecol. Prog. Ser. 234: 139-145.

 

28. Burger Y, Katzir N, Tzuri G, Portnoy V, Saar U, Shriber S, Perl-Treves R, Cohen R (2003) Variation in the response of melon genotypes to Fusarium oxysporum f. sp.melonis race 1 determined by inoculation tests and molecular markers. Plant Pathology 52: 204-211

 

29. Silberstein L, Kovalski I, Brotman Y, Perin C, Dogimont C, Pitrat M, Klingler J, Thompson G, Portnoy V, Katzir N, Perl-Treves R. Linkage map of Cucumis meloincluding horticultural traits sequence-characterized genes. Genome, 46: 761-773.

 

30. Perl-Treves R, Foley RC, Wenqiong C and Singh KB (2004) Early induction of the Arabidopsis GSTF8 promoter by specific strains of the necrotrophic fungal pathogen, Rhizoctonia solani. Molecular Plant Microbe Interactions 17: 70-80.

 

31. Tertivanidis K, Goudoula C, Vasilikiotis C, Hassiotou E, Perl-Treves R, Tsaftaris A (2004) Superoxide dismutase transgenes in sugarbeets confer resistance to oxidative agents and the fungus C-beticola. Transgenic Research 13: 225-233.

 

32. Rajagopalan P, Perl-Treves R (2005) Improved protocol for Cucumber (Cucumis sativus L.) transformation by modified explant dissection and selection. HortScience40: 431-435.

 

33. Brotman Y, Kovalski I, Dogimont C, Pitrat C, Portnoy V, Katzir N andPerl-Treves R (2005) Molecular markers linked to papaya ring spot virus resistance andFusarium race-2 resistance in melon. Theor. Appl. Genet. 110: 337-345.

 

34. Grinberg M, Perl-Treves R, Palevsky E, Shomer I, Soroker V. (2005) Interaction between cucumber plants and the broad mite, Polyphagotarsonemus latus: from damage to defense gene expression. Entomologia Experimentalis et Applicata. 115: 135-144.

 

35. Vengadesan G, Prem Anand R, Selvaraj N, Perl-Treves R, Ganapathi A. Transfer and expression of NptII and bar genes in cucumber (Cucumis sativus L.). In Vitro Cell. Dev. Biol. 41: 17-21.

 

36. Albert VA, Soltis DE, Carlson JE, Farmerie WG, Wall PK, Ilut DC, Solow TM, Mueller LA, Landherr LL, Hu Y, Buzgo M, Kim S, Yoo M-J, Frohlich MW, Perl-Treves R, Schlarbaum SE, Bliss BJ, Zhang X, Tanksley S, Oppenheimer DG, Soltis PS, Ma H, dePamphilis CW, Leebens-Mack JH. Floral gene resources from basal angiosperms for comparative genomics research. BMC Plant Biology, 5:5.

 

37. Çürük S, Çetiner S, Elman C, Xia X, Wang Y, Yeheskel A, Silberstein L, Perl-Treves R, Watad AA, Gaba V. (2005) Transformation of recalcitrant melon (Cucumis melo L.) cultivars is facilitated by wounding with carborundum. Engineering in Life Sciences, 5: 169-177.

 

38. Foley RC, Sappl PG, Perl-Treves R, Millar HA and Singh KB (2006) Desensitization of gstf8 induction by a prior chemical treatment is long lasting and operates in a tissue-dependent manner. Plant Physiology 142: 245-253.

 

39. Twito T, Weigend S, Blum S, Granevitze Z, Feldman MW, Perl-Treves R, Lavi U, Hillel J. (2007) Biodiversity of 20 chicken breeds assessed by SNPs located in gene regions. Cytogenetic and Genome Research 117: 319–326.

 

40. Herman R and Perl-Treves R. (2007) Characterization and inheritance of a new source of resistance to Fusarium oxysporum f.sp. melonis race 1.2 in Cucumis melo.Plant Disease 91: 1180-1186.

 

41. Gerchikov N, Keren-Keiserman A, Perl-Treves R, Ginzberg I. (2008) Wounding of melon fruits as a model system to study rind netting. Scientia Horticulturae 117: 115-122.

 

42. Farage-Barhom S, Burd S, Sonego L, Perl-Treves R, Lers A. (2008) Expression analysis of the BFN1 nuclease gene promoter during senescence, abscission, and programmed cell death-related processes. Journal of Experimental Botany 59: 3247-3258.

 

43. Alagarmalai J, Grinberg M, Perl-Treves R, Soroker V (2009) Host selection by herbivorous mite Polyphagotarsonemus latus (Acari: Tarsonemidae). J. Insect Behaviour22:375–387.

 

44. Boualem A, Troadec C, Kovalski I, Sari MA, Perl-Treves R and Bendahmane A (2009) A conserved Ethylene Biosynthesis Enzyme Leads to Andromonoecy in twoCucumis species. PLoS One 4: e6144.

 

45. Chatzidimitriadou K, Nianiou-Obeidat I, Madesis P, Perl-Treves R, Tsaftaris A (2009) Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration. Electronic Journal of Biotechnology 12: 10.

 

46. Zvirin Z, Herman R, Brotman Y, Denisov Y, Belausov E, Freeman S and Perl-Treves R (2010) Differential colonization and defense responses of resistant and susceptible melon lines infected by Fusarium oxysporum race 1.2. Plant Pathology 59, 576–585.

 

47. Twito T, Madeleine D, Perl-Treves R, Hillel J, Lavi U (2011) Comparative genome analysis with the human genome reveals chicken genes associated with fatness and body weight. Animal Genetics 42: 642-649

 

48. Brotman Y, Normantovich M, Goldenberg Z, Zvirin Z, Kovalski I, Stovbun N, Donniger T, Bolger AM, Troadec C, Bendahmane A, Cohen R, Katzir N, Pitrat M,Dogimont C, Perl-Treves R (2013) Dual resistance of melon to Fusarium oxysporum races 0 and 2 and to Papaya ring-spot virus is controlled by a pair of head-to-head oriented NB-LRR genes of unusual architecture. Molecular Plant 6: 235-238 Supplemental data

 

Books Invited Reviews and Book Chapters

 

1. Leshem YY, Kuiper PJC, Erdei L, Lurie S and Perl-Treves R. (1998) Do Selye’s mammalian “GAS” concept (General Adaptation Syndrome) and “Co-stress” response exist in plants? In: Stress of Life: from Molecules to Man. Editor: P. Csermely. Annals of the New York Academy of Science, vol. 851, pp. 199-208.

 

2. Perl-Treves R (1999) Male to female conversion along the cucumber shoot: approaches to study sex genes and floral development in Cucumis sativus. In: Sex Determination in Plants. Editor: C.C. Ainsworth. BIOS Scientific Publisher, Oxford, pp. 189-216.

 

3. Perl-Treves R (1999) Floral development and sex expression in Cucumis sativus, the cucumber: genetic and molecular approaches. Flowering Newsletter 28: 39-48.

 

4. Perl-Treves R. and Perl A. (2002) Oxidative stress: an introduction. In: Oxidative Stress in Plants. Van Montagu M., Inze D., Taylor and Francis Books Ltd, London andNew York.

 

5. Perl-Treves R and Rajagopalan P (2006) Close, yet separate: patterns of male and female floral development in monoecious species. In: Flowering and its Manipulation. Editor: C.C. Ainsworth. Blackwell, Oxford, pp. 117-146.

 

6. Oumouloud A, El-Otmani M, González Torres R, Garcés Claver A,Perl-Treves R, Álvarez JM (2013) Breeding melon for resistance to Fusarium wilt: Recent developments. Euphytica, in press

 

Selected Chapters in Proceedings Books

 

1. Rajagopalan P, Saraf-Levy T, Lizhe A and Perl-Treves, R (2004) Over-expression of an ethylene receptor increases femaleness in transgenic cucumbers. Proceedings of the VIIIth EUCARPIA Meeting on Cucurbit Genetics and Breeding, Olomouc, Czech Republic, 12-17 July 2004, 525-531.

 

2. Brotman Y, Kovalski I, Dogimont C, Pitrat M, Katzir N and Perl-Treves R (2004) Molecular mapping of the melon Fom-1/Prv locus. Proceedings of the VIIIthEUCARPIA Meeting on Cucurbit Genetics and Breeding, Olomouc, Czech Republic, 12-17 July 2004, pp. 485-489.

 

3. Grinberg G, Soroker V, Palevsky E, Shomer I and Perl-Treves R(2004) Response of cucumber to the broad mite, Polyphagotarsonemus latus. Proceedings of the VIIIthEUCARPIA Meeting on Cucurbit Genetics and Breeding, Olomouc, Czech Republic, 12-17 July 2004, 319-324.

 

4. Herman R, Zvirin Z, Kovalski I, Freeman S, Denisov Y, Zuri G, Katzir N,Perl-Treves R (2008) characterization of Fusarium race 1.2 resistance in melon and mapping of a major QTL for this trait near a fruit netting locus. Proceedings of the IXth EUCARPIA Meeting on Cucurbit Genetics and Breeding, Avignon, France, 21-24 May 2008, 149-156.

 

5. Baniel A, Saraf-Levy T, Perl-Treves R. (2008) How Does the First Fruit Inhibit Younger Fruit Set In Cucurbits? Proceedings of the IXth EUCARPIA Meeting on Cucurbit Genetics and Breeding, Avignon, France, 21-24 May 2008, 597-601.

 

6. Valentina Davidovich V, Leibman D, Gal-On A, Perl-Treves R (2008) Affecting cucurbit hormonal status by the ZYMV transient expression vector. Proceedings of theIXth EUCARPIA Meeting on Cucurbit Genetics and Breeding, Avignon, France, 21-24 May 2008, 587-591.

 

7. Fraenkel R , I. Kovalski I, C. Troadec C, Bendahmane A, Perl-Treves R (2012) A TILLING population for cucumber forward and reverse genetics. In: Proceedings of the X Eucarpia Meeting on the Cucurbitaceae, Antalia, October15-18 2012, pp, 598-603.

 

8. Miller G, Goltsev Y, Dalia Wolf D, Baniel A, Saraf-Levy T, Kovalski I,Perl-Treves R (2012) Developmental changes in inhibited, senescent and fruit-setting ovaries of cucumber. In: Proceedings of the X Eucarpia Meeting on the Cucurbitaceae, Antalia, October15-18 2012, pp, 591-596.

 

9. Normantovich M, Yogev O, Taylor CG, Perl-Treves R (2012) Study of the Fom-2 resistance gene using composite melon plants. In: Proceedings of the X Eucarpia Meeting on the Cucurbitaceae, Antalia, October15-18 2012, pp, 240-246. 

 

מחקר

* שים לב: בגירסה האנגלית של האתר מובא פירוט מלא ומאוייר של מחקרינו.

תחום הגנטיקה המולקולרית בצמחים הוא מעניין ודינמי, גם מבחינה מדעית וגם מהצד היישומי. מעבדתנו מתמחה בצמחים ממשפחת הדלועיים - בעיקר מילון ומלפפון. הפרוייקטים השונים עוסקים בעיקר בשני נושאים:

א)    כיצד מתגונן הצמח מפני מחלות ומזיקים? במסגרת זו אנו מבצעים:

1)  מיפוי ושיבוט הגן לעמידות לפטריית הפוזריום גזע 2 (גזע 0) במלון, התוקפת את השורשים ומחוללת מחלת נבילה קשה. כן נעשה מיפוי ושיבוט הגן לעמידות לווירוס הפאפאיה ווירוס צהבון הקישוא במלון. המיפוי נעשה בעזרת סמני דנ"א שאנו מפתחים או מיישמים במיגוון של שיטות, ובעזרת אוכלוסיות צמחים בהן מתפצלת העמידות למחלות הנ"ל כתכונה מונוגנית-דומיננטית. מיפוי כזה מספק למטפחי הזנים סמני דנ"א דיאגנוסטיים לאיתור תכונת העמידות בצאצאי ההכלאות שהם מבצעים, והוא הוביל גם לשיבוט הגן לעמידות והבנת דרך פעולתו. בעקבות כך אנו חוקרים את האינטראקציה החלבונית בין חלבוני עמידות לחלבונים של הפתוגן ("אפקטורים). כן ביצענו איפיון גנטי ומיפוי של עמידות לפוזריום גזע 1.2 במלון. זהו גזע נוסף של אותה פטריה אך מנגנון ההורשה של העמידות כמותי, מעורבים בו כנראה מספר גנים.

2) זיהוי מנגנוני הגנה של צמחים כנגד אקרית העיוותים - פרוק-רגליים מיקרוסקופי התוקף טווח גידולים רחב מאוד, ומעט ידוע על תגובת הצמח ודרכי הגנה אפשריות מפניו. אנו מאפיינים את מנגנון הנזק ואת התגובה של הצמח ברמה האנטומית, הביוכימית, וביטוי הגנים, עקב ההתקפה.

ב) כיצד מבקרים בני משפחת הדלועיים את התמיינות פרחי הזכר והנקבה על הצמח? דלועיים מצטיינים במנגנוני התפתחות ייחודיים ומורכבים המווסתים את מין הפרחים. הללו מעניינים גם מבחינה ביולוגית-התפתחותית, גם בגלל חשיבותם בקביעת היבול ובשליטה על ההאבקה. מעבדתנו שיבטה גנים קובעי-מין במלפפון וגילתה כי גנים אלה קשורים לייצור ממוקד של ההורמון אתילן בניצן הפרח.

ג) כיצד מבוקר מספר הפירות שצמח עשוי לחנוט? מדוע חלק מהשחלות המופרות אינן חונטות פרי? אילו הורמונים מבקרים את התהליך? נושא זה נחקר במגוון שיטות כגון צמחים מהונדסים, מוטנטים בגנים של מסלולי הסוכר וההורמונים, ושיטות מעולם הביולוגיה המערכתית.  

ד) לימוד השונות הגנטית במין מלון, מין בעל גיוון עצום בתכונות הפרי. בפרוייקט משותף ישראלי-פלסטיני אנו יוצרים ומאפיינים אוסף של זנים מקומיים המהווים משאב גנטי לטיפוח זנים עתידי.

 

קורסים

Course # 80-701 INTRODUCTION TO BIOLOGY

2 hrs lecture, 1st year, 1st semester.

 

Provides an introduction to biological principles and concepts such as membranes, biological macromolecules, prokaryotic and eukaryotic cells, multicellularity, genetic information,. An overview of bacteria, viruses, fungi and protists; a taxonomic survey of the plant kingdom from lower to higher plants. Major plant processes: photosynthesis, nitrogen assimilation, plant-water relationships, phloem transport, response to environmental stimuli.

 

 

Course No. 80-225 PLANT PHYSIOLOGY

Lecture: 3 hours a week, 2nd year, 2nd semester (jointly with Dr. Orit Shaul).

 

The course covers major topics in Plant Biochemistry and Physiology. The scientific approaches and techniques that served to make the main discoveries in the field are emphasized. Relevance of physiological processes to Agriculture and Environmental Protection is discussed, and examples of Molecular studies based on Plant Physiology are given.

Contents: Introduction: The Plant Kingdom, vegetal "principles of life", challenges of botanical research. Photosynthesis: thermodynamic and photochemical principles, light and dark reactions, chloroplast structure, photorespiration, adaptation to environmental changes, sucrose and starch synthesis. Plant-water relations: water potential and its components, water and mineral conduction in the soil-plant-atmosphere continuum, the photosynthesis-transpiration dilemma, stomatal action, response to drought stress. Transport of assimilates in the phloem. Nitrogen metabolism in plants. Introduction to growth and differentiation. Plant hormones: structure, assays, responses, mode of action. Photomorphogenesis: phytochrome and other photoreceptors, photoperiodic induction of flowering.

 

 

Course # 80-329 PRINCIPLES OF PLANT MOLECULAR GENETICS AND BIOTECHNOLOGY

2 hrs lecture + 2 hrs practice, 3rd year, 2nd semester

 

Key-aspects of Plant Genetics are addressed, starting from the classical genetic level, required to understand the foundation of the more modern approaches, through somatic cell genetics and cytogenetics, down to molecular studies of the Plant Genome and Genetic Engineering.

Contents: Introduction to the plant genome: nuclear genome size and composition, genomics approaches. The chromosomes: ploidy systems in plants, cytogenetic achievements in plant research and breeding. The chloroplast and the mitochondrial genomes in plants. Genome mapping and use of molecular markers in plant breeding. Map-based cloning. Plant tissue culture and its applications: protoplast and cell cultures, embryogenesis and organogenesis, haploid cultures, in vitro selection. Somatic cell fusions. Genetic engineering of plants: transformation methods. Overexpression and repression of plant genes; marker genes and reporter genes; examples of transgenic plants with agricultural potential.

Practice involves reading and analysis of relevant scientific literature, genomics database mining and computing a genetic map, and visits to a plant tissue culture company and a seed company that applies molecular methods in breeding.

 

 

Course # 80-491 UNDERGRADUATE STUDENTS SEMINAR    

 

The course consist of 1-hour seminars, a series of presentations and discussions delivered by students on a current research topic in Plant Biology.

 

MOLECULAR AND HORMONAL REGULATION OF PLANT DEVELOPMENT  (new Course planned for 2013-2014)

2 hrs lecture, jointly with Dr. Hinanit Koltai and Dr. Vered Irihimovitch, ARO

 

 The course discusses major themes in plant development, related to leaf, root, flower and fruit induction and organogenesis. Hormonal signals and molecular mechanisms that orchestrate plant development are emphasized. Students are requested to present mini-seminars related to key research articles, as a basis for in depth discussion of modern methodologies and model plants employed in the field.