Prof. Barda-Saad Mira

Prof.
Associate Professor
Prof. Mira Barda-Saad
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CV

Education

 

1984-1987, BSccum laude

Bar-Ilan University, Ramat-Gan, Israel

 

1988-1990, MSccum laude, Experimental Hematology and Microbiology

Bar-Ilan University, Ramat-Gan, Israel

 

1993-1999, PhDsumma cum laude, Molecular and Cellular Biology

Bar-Ilan University, Ramat-Gan, Israel

 

1999-2001, POSTDOCTORAL FELLOW, Molecular Immunology

Weizmann Institute of Science, Rehovot, Israel

Advisor: Prof. Dov Zipori

 

2001-2006, VISITING FELLOW

Molecular Immunology and Signal Transduction

Laboratory of Cellular and Molecular Biology

National Cancer Institute, NIH

Bethesda, MD, USA

Advisor: Dr. Larry Samelson

 

 

Positions

 

2013-present, ASSOCIATE PROFESSOR

The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University

Ramat-Gan, Israel

 

2006-2013, SENIOR LECTURER

The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University

Ramat-Gan, Israel

 

1993-1996, TEACHING ASSISTANT

Bar-Ilan University, Ramat-Gan, Israel

 

1990-1993, RESEARCH FELLOW

Bar-Ilan University, Ramat-Gan, Israel

 

1988-1990, TEACHING ASSISTANT

Bar-Ilan University, Ramat-Gan, Israel

 

International Recognition

 

1987, Dean’s award for excellence, Bar-Ilan University, Israel

 

1988, Special excellence award from the Israeli Knesset (Parliament), Israel

 

2004, FARE Award (Fellow Award for Research Excellency), NIH, Bethesda, MD, USA

 

2008, Research Award for Excellence, Israeli Cancer Association, Israel

 

2009, Leon and Maria Taubenblatt award for Excellence in Medical Research

 

   

Publications

  1. Barda-Saad, M. Sredni, D., Zemer, R., Lenz, R. and Rozensajn L.A. (1990). T-lymphocyte colony enhancing factor. Harefuah 118: 92-5.
  2. Barda-Saad, M., Zipori, D., Globerson, A., Zhang, A.S. and Rozenszajn, L.A. (1996). Selective adhesion of immature thymocytes to bone marrow stromal cells: relevance to T cell lymphopoiesis.Exp Hematol. 24: 386-91.
  3. Barda-Saad, M., Zhang, A.S., Zipori, D. and Rozenszajn, L.A. (1997). Adhesion of thymocytes to bone marrow stroma cells: regulation by bFGF and IFN-gamma. Stem Cells 15: 229-36.
  4. Yu, S., Wang, S.L., Zhang, H.W., Huang, Z., Abel, L., Barda-Saad, M., Zipori, D. and Globerson, A. (1998). Effect of aging on the expression of surface antigen in mouse thymocytes. Acta Zoologica Sinica, 44:466-474.
  5. Barda-Saad, M., Rozenszajn, LA, Ashush, H., Shav-Tal, Y., Ben-Nun, A. and Zipori, D. (1999). Adhesion molecules involved in the interactions between early T-cells and mesenchymal bone marrow stromal cells. Exp Hematol. 27: 834-44.
  6. Ashush, H., Rozenszajn, LA, Blass, M., Barda-Saad, M., Azimov, D., Radnay, J., Zipori, D. and Rosenschein U. (2000). Apoptosis induction of human myeloid leukemic cells by ultrasound exposure. Cancer Res. 60:1014-20.
  7. Zipori, D., Barda-Saad, M. (2001). Role of activin A in negative regulation of normal and tumor B lymphocytes. J. Leukoc. Biol. 69:867-73.
  8. Shoham, T., Sternberg, D., Brosh, N., Barda-Saad, M. and Zipori D. (2001). The promotion of plasmacytoma growth by mesenchymal stroma is antagonized by basic fibroblast growth factor induced activin A. Leukemia 15:1102-10.
  9. Barda-Saad, M., Shav-Tal, Y., Rozenszajn, L.A., Cohen, M., Zauberman, A., Karmazyn, A., Parameswaran, R, Schori, H, Ashush, H., Ben-Nun, A. and Zipori D. (2002). The mesenchyme expresses T cell receptor mRNAs: relevance to cell growth control. Oncogene 21:2029-36.
  10. Shoham, T., Parameswaran, R., Shav-Tal, Y., Barda-Saad, M., and Zipori D. (2003). The Mesenchymal stroma negatively regulates B cell lymphopoiesis through the expression of activin A.Ann. N Y Acad. Sci. 996:245-260.
  11. Barda-Saad, M.,  Braiman, A.,  Titerence, R.,   Bunnell, S.C.,   Barr, V.A.,  and Samelson,  L.E. (2005) Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton.Nature Immunology 6:80-89.
  12. Houtman, J.C., Houghtling, R., Barda-Saad, M., Toda, Y., Schwartzberg, PL., and Samelson, LE. (2005). Early phosphorylation kinetics of proteins involved in proximal T-cell receptor-mediated signaling pathways. J. Immunol. 15:2449-2458.
  13. Houtman, J.C., Barda-Saad, M. and Samelson, L.E. (2005). Examining multiprotein signaling complexes from all angles. The use of complementary techniques to characterize complex formation at the adapter protein LAT. FEBS J. 272:5426-35.
  14. Barda-Saad, M., Braiman, A., Titerence, R., Barr, V. and Samelson, L.E. (2006). T cell antigen receptor engagement induced cytoskeleton re-organization. CCR Frontiers in Science (Published by the National Cancer Institute’s Center for Cancer Research, NIH).
  15. Braiman, A., Barda-Saad, M. and Samelson, L.E. (2006). Recruitment and activation of PLCg1in T-cells: a new insight into old domains. EMBO J. 25:774-84.
  16. Barr, V.A., Balagopalan-Bhise, L., Barda-Saad, M., Polishchuk, R., Boukari, H.,
    Bunnell, S.C., Bernot, K.M., Toda, Y., Nossal, R., and Samelson,  L.E. (2006). T-cell antigen receptor-induced signaling complexes: Internalization via a cholesterol-dependent endocytic pathway. Traffic 7:1143-1163.
  17. Houtman, J.C., Yamaguchi, H., Barda-Saad, M., Bowden, B., Schuck, P., Appella, E., and Samelson, LE. (2006). Oligomerization of signaling complexes by the multipoint binding of Grb-2 to both LAT and Sos1. Nature Struct. Mol. Biol. 13:798-805.
  18. Balagopalan, L., Barr, V.A., Sommers, C.L., Barda-Saad, M., Goyal, A., Isakowitz, M.S. and Samelson, L.E. (2007). c-Cbl mediated regulation of LAT-nucleated signaling complexes. Mol. Cell. Biol. 27:8622-8636.
  19. Sommers, C., Gurson, J.M., Surana, R., Barda-Saad, M., Lee, J., Kishor, A., Li, W.,
    Gasser, A.J., Barr, V., Miyaji, M., Love, P. and Samelson, L.E. (2008) Bam-32: a novel mediator of Erk activation in T cells. Int. Immunol. 20:811-818.
  20. Shani N, Rubin-Lifshitz, H., Sccolnick, K., Peretz-Chohen Y. Shkolnik, K., Shnider, V., Cohen, M., Shav-Tal, Y., Barda-Saad, M. and Zipori D. (2009) Incomplete T-cell receptor-beta peptides target the mitochondrion and induce apoptosis. Blood. 9:3530-3541.
  21. Barda-Saad, M, Shirasu, N., Pauker M.H, Hasan, N., Perl, O., Balbo, A., Yamaguchi, H., Houtman, J., Appella, E., Schuck, P. and Samelson, LE. (2010) Cooperative interactions at the SLP-76 complex are critical for actin polymerization. EMBO J. 29:2315-2328Single Corresponding author
  22. Reicher, B. and Barda-Saad, M. (2010). Multiple Pathways leading from the T-cell antigen receptor to the actin cytoskeleton network. FEBS Lett. 584:4858-4864.  Single Corresponding author
  23. Hazani, M. and Barda-Saad, M. (2010). Studies of novel interactions between Nck and VAV SH3 domains. Comm. Integ. Biol. 4:175-177Invited, Single Corresponding author
  24. Pauker, H.M., Reicher, B., Fried, S., Perl, O., and Barda-Saad, M. (2011) Functional cooperation between the proteins Nck and ADAP is fundamental for actin reorganization. Mol Cell Biol. 31;2653-2666.  Single Corresponding author
  25. Pauker, H.M., Hassan, N. Noy, E. Reicher, B. and Barda-Saad, M. (2012) Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET.Science Signaling 5(221):rs3 (cover). Single Corresponding author
  26. Reicher, B., Joseph, N., David, A., Perl, O. and Barda-Saad, M. (2012) Ubiquitylation-dependent negative regulation of WASp is essential for actin cytoskeleton dynamics. Mol. Cell. Biol. 32:3153.Single Corresponding author
  27. Noy, E., Fried, S. Matalon, O. and Barda-Saad M. (2012) WIP remodeling actin behind the scenes: How WIP reshapes immune and other functions. Int. J. Mol. Sci. 13:7629. Single Corresponding author
  28. Reicher, B., Perl, O., Matalon, O. and Barda-Saad, M. (2012) WASp and WAVE proteins: from structure, through function, to clinical aspects. Review, J. Clin. Cell. Immunol.,http://dx.doi.org/10.4172/2155-9899.S12-001Single Corresponding author
  29. Pauker, H.M., Noy, E. and Barda-Saad, M. (2013) Following the dynamics of signaling protein interactions in live activated T cells. JoVEhttp://www.jove.com/video/50076Single Corresponding author
  30. Ovadia, A., Livneh, A., Feld, O., Ben-Zvi, I., Kukuy, E., Kivity, S., Lidar, M., Barda-Saad, M. and Shinar, Y. (2013) T helper 17 polarization in familial Mediterranean fever. Genes & Immun. 1-5. doi: 10.1038/gene.2013.6.
  31. Joseph, N., Reicher, B. and Barda-Saad, M. (2013) The calcium feedback loop and T-cell activation: how the cytoskeleton networks control intracellular calcium levels. Biochimica et Biophysica Acta (BBA) – BiomembranesInvited, 105:481-93. Single Corresponding author
  32. Reicher, B., Matalon, O. and Barda-Saad, M. (2013) WASp function in T cell signaling microclusters. Immunol. RevInvited, 256:10-29. Single Corresponding author
  33. Haba, N.Y., Gross, R., Novacek, J., Shaked, H., Zidek, L., Barda-Saad, M. and Chill, J.H. (2013) NMR determines transient structure and dynamics in the disordered C-terminal domain of WASp interacting protein. Biophys. J. 105:481.
  34. Klieger, Y., Almogi-Hazan, O., Ish-Shalom, E., Pato, A., Pauker, H.M., Barda-Saad, M., Wang, L.  and Baniyash, M. (2013) Unique ζ-chain motifs mediate a direct TCR-actin linkage critical for immunological synapse formation and T-cell activation. Eur. J. Immunol. 44:58.
  35. Fried, S., Pauker, M. H., Reicher, B., Eliyahu, S., Matalon, O., Noy, E., Chill, J., and Barda-Saad, M. Conformational changes within the WIP:WASp actin-regulating complex: a triple-color FRET study.Science Signaling. Under Revision.  Single Corresponding author
  36. Elazari-Shalom, H., Shaked, H., Esteban-Martin, S., Salvatella, X., Barda-Saad, M. and Chill, J. (2014) NMR investigation of transient structure in the disordered N-terminal domain of WASp interacting protein. The FEBS JournalUnder revision.
  37. Borodianskiy-Shteinberg, T., Kalt, I., Kipper, S., Katz, S., Pauker, H. M., Barda-Saad, M., Gerber, D., and Sarid, R. (2014) The Nucleolar PICT-1/GLTSCR2 Protein Forms Homo-Oligomers. Journal of Molecular BiologyIn Press.
  38. Fried, S., Matalon, O., Noy, E., and Barda-Saad, M. (2014) WIP: More than a WASp interacting protein. Journal of leukocyte biologyUnder Revision.  Single Corresponding author
  39. Joseph, N., Reicher, B., Ahuvit D., Matalon, O., and Barda-Saad, M. (2014) Ubiquitylation-dependent downregulation of Nck  regulates its functional activity. FEBS Lett. 588:3808.
  40. Pauker, MH., Reicher, B., Wortzel1, I., Joseph, N., Jakubowicz, S., Perl, O., and Barda-Saad, M. (2014) WASp Family Verprolin-Homologous Protein-2 (WAVE2) and Wiskott-Aldrich Syndrome Protein (WASp) Engage in Distinct Downstream Signaling Interactions at the T Cell Antigen Receptor Site. JBC, 289:34503.
  41. Elazari-Shalom, H., Shaked, H., Esteban-Martin, S., Salvatella, X., Barda-Saad, M. and Chill, J. (2015) New insights into the role of the disordered WIP N-terminal domain revealed by NMR structural characterization. The FEBS Journal, 282:700.
  42. Ziv-Av, A., Giladi, ND., Lee, HK., Cazacu, S., Finniss, S., Xiang, C., Pauker, H.M., Barda-Saad, M., Poisson, L. and Brodie, C. (2015) RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK. Oncotarget,  6:19826.
  43. Matalon, O., Fried, S., Ben-Shmuel, A., Pauker, H.M., Joseph, N., Keizer, D., Piterburg, M. and Barda-Saad, M. (2016) NK cell cytotoxicity is regulated by LAT and PLCgamma which serve as novel substrates of SHP-1. Science Signaling, 9 (429): ra54.

Chapters in Books

  1. Reicher, B., and Barda-Saad, M. (2011) Invited Book’s Chapter: The molecular mechanisms of actin regulatory proteins. Chapter 6 in: Actin: Structure, Functions and Disease (Online book edition). Eds: Victoria A. Consuelas and Daniel J. Minas. Nova Science Publishers, Inc.
  2. Elad, N., Reicher, B. and Barda-Saad, M. (2011) Invited Book’s Chapter: Stoichiometry of Signaling Complexes in Immune Cells: Regulation by the Numbers. Chapter 9 in: Stoichiometry and Research- The importance of quantity in biomedicine (Online book edition). Ed: Alessio Innocenti,InTech publications, Inc. 

Patents

  1. PCT/IL02/00130- #20040259196, T Cell Receptor variants expressed in Mesenchymal cells and uses thereof. Pending.
  2. PCT/IL02/00129 - #20040101931, Immunoglobulin Superfamily variants expressed in Mesenchymal cells and therapeutic uses thereof. US Pending. EP 1389236.
  3. #20070196365 – Immunoglobulin heavy chain variants expressed in mesenchymal cells and therapeutic uses thereof. Pending.

Courses

80-385-01 CONTROL MECHANISMS OF THE IMMUNE RESPONSE

Thursday, 14:00-16:00, 2nd semester

 

80-210-01 /10 INTRODUCTION TO IMMUNOLOGY

Sunday, 10:00-12:00, 2nd semester

Tuesday, 16:00-18:00, 2nd semester

 

80-930-01 SEMINAR (A)

Friday, 10:00-11:00, 1st semester

 

80-493-01 SEMINAR (B)

Friday, 10:00-12:00, 2nd semester