Prof. Ron Goldstein

Prof.
Full Professor
Prof. Ron Goldstein
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CV

Education

1974-1978, BA, Biology, Columbia College (NY)

1978-1981, MA, Neurobiology, Columbia University

1981-1984, PhD, Neurobiology and Behavior, Columbia University

Thesis adviser: James H. Schwartz

1983-1984, Lady Davis Postdoctoral fellow

Neurobiology& Behavio, Dept. of Zoology, Hebrew University

1984-1991, Postdoctoral Fellow

Developmental Neurobiology, Depts. of Anatomy and Embryology/Zoology Hebrew University

Academic Positions

1991-2003, SENIOR LECTURER

Dept. Life Sciences, Bar-Ilan University

2003-2011, ASSOCIATE PROFESSOR

The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University

2011-present PROFESSOR

The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University

Research Group

 

Noy Elmaliach, MSc. Student

Laura Malka M.Sc., Research worker

Dr. Amos Markus, post-doctoral fellow

Dr.Tatiana Borodianskiy Shteinberg, post-doctoral fellow

Dr. Dana Berneman-Zeitooni, Lab manager

Current Funding

IsraelScience Foundation              2016-2020

National Institutes of Health            2015-2020

(with P. Kinchington, Univ. Pittsburgh)

US-Israel Binational Foundation  2014-2018

Maximillian Goode Foundation

Publications

Selected Publications

Human embryonic stem cell-derived neurons for studying human neurotropic viruses

Markus, A., Lebenthal-Loinger, I., Yang, I.H., Kinchington, P.H. and Goldstein, R.S. An in-vitro model of latency and reactivation of varicella zoster virus in human stem cell-derived neurons by PLOS Pathogens 2015
 
Grigoryan, S. Yee, MB Glick, Y, Gerber, D, Kepten, E, Garini, Y, Yang, IY Kinchington, PR and Goldstein R.S. Fusion mediated direct transfer of viral and cellular proteins from Varicella-Zoster Virus-infected non-neuronal cells to human neurons. PLOS One 2015

Sloutskin, A, Yee, MB, Kinchington, PR and Goldstein, RS Varicella zoster virus and herpes simplex virus type 1 can infect and replicate in the same neurons whether co- or superinfected. J Virol. 2014 88:5079-86


Markus, A., Waldman Ben-Asher, H., Kinchington,P.R. Goldstein, RS  Cellular transcriptome analysis reveals differential expression of pro- and anti-apoptosis genes by VZV-infected neurons and fibroblasts . J. Virol, 2014 88: 7674 –7677.

Birenboim, R. Markus, A. Goldstein, R. S. Simple generation of neurons from human embryonic stem cells using agarose multiwell dishes J. Neurosci Methods 214:9-14 2013

Grigoryan, S., Kinchington, P.R., Yang, I.H., Selariu, A., Zhu, H., Yee, M., and Goldstein, R.S. Retrograde axonal transport of VZV: live-imaging studies in hESC-derived neurons J. NeuroVirol. 18:462-470 2012

Dukhovny, A. Sloutskin, A., Markus, A. Yee, M., Kinchington, P.K. and Goldstein, R.S. VZV infects human embryonic stem cell-derived neurons and neurospheres, but not pluripotent embryonic stem cells or early progenitors. J. Virology. 86:3211-3218, 2012. (Spotlighted article)

Markus, A., Grigoryan, S., Sloutskin, A., Yee, M.B., Zhu, H, Yang, I-H., Thakor,N., Sarid, R.,  Kinchington, P.R.  and Goldstein, R.S. Varicella zoster virus infection of neurons derived from human embryonic stem cells: direct demonstration of axonal infection, transport of VZV and productive neuronal infection.  J. Virol. 85:6220-33 2011

Differentiation of human embryonic stem cells to neural derivatives

Ziegler, L., Grigoryan, S. Yang, I-H, Thakor,N and Goldstein, R.S. Efficient derivation of Schwann cells from human embryonic stem cells (original article).Stem Cell Reviews and Reports, 7:394-403 2011

Ziegler,L., Segal-Ruder,Y., Coppola, G., Geschwind, D., Fainzilber,M., and Goldstein,R.S. A human neuron injury model for molecular studies of axonal regeneration  Exper. Neurol  223:119-127 2010

Goldstein, R.S. Transplantation of mammalian embryonic stem cells and their derivatives to avian embryos. Stem Cell Reviews and Reports (invited review) 6:473-484, 2010

Brokhman, I., Pomp, O. Fishman, L, Tennenbaum, T., Amit, M., Itzkovitz-Eldor, J. and Goldstein, R.S. Genetic modification of human embryonic stem cells with adenoviral vectors: heterogeneity of infectability between lines and increased infectability with differentiation correlates with expression of the coxsackie virus receptorStem Cells and Development 18:447-456 2009

Pomp, O., Brokhman, I., Gamarnik, L, Almog, M., Korngreen, A., Tavian, M., and Goldstein,R.S. PA6-induced human embryonic stem cell-derived neurospheres: a new source of human peripheral sensory neurons and neural crest cells Brain Research 1230:50-60 2008 (cover photo)

Pomp, O., Brukhman, I., Ben-Dor, I., Reubinoff, B. and Goldstein, R.S. Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells. Stem Cells 23:923-930, 2005.

Goldstein, R.S., Drukker, M. Reubinoff, B.E. and Benvenisty, N. Integration and differentiation of human embryonic stem cells transplanted to the chick embryo. Dev. Dynam.  225:80-86, 2002.

Schuldiner, M., Eiges,R., Eden,A., Yanuka, O., Itskovitz-Eldor,J., Goldstein, R.S.and Benvenisty, N. Induced neuronal differentiation of human embryonic stem cells Brain Res. 913:201-205, 2001 (cover photo, 350 + citations).

Biotechnological uses of the chick embryo

Grinberg,I., Duchovny, A. and Goldstein, R.S.Whole-animal imaging of human blood malignancy in turkey embryos: visualization of homing and engraftment. Leukemia and Lymphoma, 53:472-8 2012

Farnoushi, Y., Cipok,M., Kay, S., Naparstek,E., Goldstein,R.S. and Deutsch,V.R. Rapid in vivo testing of drug response in multiple myeloma made possible by xenograft to turkey embryos Br. J. Cancer, 105:1708-1718 2011

Noiman, T. Buzhor, E., Metsuyanim, S. Harari-Steinberg, O., Morgenstern, C., Dekel, B and Goldstein, R.S. A rapid in-vivo assay system for analyzing the organogenetic capacity of human kidney cells Organogenesis, 7:140-144 2011

Slobdobnik, D. Grinberg, I, and Goldstein, R.S. Human skin transplanted to the chick chorioallantoic membrane for prediction of contact hypersensitivity: an alternative to murine local lymph node assay. Experimental Dermatology 18:409-413 2009

Grinberg,I., Reis, A. Ohana, Taizi, M, Rund, D., Cipok, M., Tavor, S., Deutsch, V. and Goldstein, R.S. Engraftment of human blood malignancies to the turkey embryo: a robust, rapid and inexpensive in-vivo model. Leukemia Research 33:1417-1426 2009

Taizi, M., Deutsch, V.R., Leitner, A., Ohana, A. and Goldstein, R.S. A rapid in-vivo system for studying and testing therapeutics on human blood malignancies. Exp. Hematol. 34:1698-1708, 2006.

Early vertebrate development (chick embryo)

Goldstein, R.S. and Kant, R. Plasticity of axial identity among somites: cranial somites can generate vertebrae without expressing Hox genes appropriate to the trunk. Dev. Biol. 216:507-520, 1999 (cover photo, Jan 2000).

Goldstein, R.S., Teillet, M. A. and Kalcheim, C. Microenvironment created by grafting rostral half-somites is mitogenic for neural crest cells. Proc. Nat. Acad. Sci. (USA) 12:4476-4480, 1990.

Goldstein, R.S. and Kalcheim, C. Determination of epithelial half-somites in skeletal morphogenesis. Development116:441-445, 1992.

Patents

Methods for generating neuronal cells from human embryonic stem cells and uses thereof (with Reubinoff, B.) US Patent application filed April 8, 2005, awarded Singapore, 2009.

Chimeric avian-based screening system containing mammalian grafts (with Tennenbaum Tamar and Deutsch Varda) PCT Publication Date: May 1, 2006. USPTO publication April 2009

Research

Neurons derived from human embryonic stem cells (hESC) for studying pathogenic viruses

The differentiation of human embryonic stem cells into neural derivatives and applications in biomedicine has been the central focus of our research since 2001.

Our lab was the first to generate primary sensory (DRG-like) neurons and their precursors, neural crest cells, from hESC (Pomp et al Stem Cells, 2005, international patents pending and awarded). Since then, we have steadily improved the yield of this neuronal phenotype in our cultures (Brokhman et al, 2008, Pomp et al, 2008). The efficient generation of human neurons now allows us to use them in two biomedical applications.

The primary research focus in the lab is now using human neurons to study infection by pathogenic neurotropic viruses, i.e. varicella zoster ("chicken pox/shingles"). This project is a close collaboration with Prof. Paul Kinchington from the the Universityof Pittsburgh Medical School.

Courses

80-414 BIOTECHNOLOGY SEMINAR

80-545  BIOTECHNOLOGICAL USES OF STEM CELLS

80-849 IMAGE PROCESSING FOR BIOLOGISTS

              INTRODUCTION TO BIOLOGY B (English for Post-bac Premed program)

80-902 GRADUATE SEMINAR (A)